| Sample Material |
Measure out 5.00g sample material with a universal balance and put it into a centrifugal tube. |
| |
30ml acetonitrile |
| |
Stir it for 1 minute with a rotary evaporator. |
| |
Clean the rotation axis with some acetonitrile. |
| |
Centrifugation at speed of 2,500r/min for 5 minutes. |
| |
| Acetonitrile Layer |
|
|
|
| |
Cotton Cork
Filtration |
Residue |
|
| |
|
|
| |
|
| |
Stir it for 1 minute with a rotary evaporator. |
|
| |
|
| |
Clean the rotation axis with some acetonitrile. |
|
| |
|
| |
Centrifugation at speed of 2,500r/min for 5 minutes. |
|
| |
| |
|
Acetonitrile Layer |
|
| |
|
| |
Cotton Cork Filtration |
|
Residue |
|
| |
| |
60ml acetonitrile and saturation ethane respectively. |
| |
Vibrate it for 5 minutes. |
| |
| Acetonitrile Layer |
Ethane Layer |
|
| |
10ml propyl alcohol |
| |
Decompression & condensation £¨ below 40 ¡æ £©£¬ dry solidification ( nitrogen flow £© |
| |
Methyl-alcohol-and-water mixed liquor (1 £º 1) (contain 0.1 £¥ vitamin C) 5ml transfer pipet |
| |
(Dissolve by ultrasound, MESOBURAIRUTA0.45um) filtration |
| |
|
|
|
| High Performance Liquid Chromatograph |
|
